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KMID : 0380020030180040301
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Korean Journal of Biotechnology and Bioengineering
2003 Volume.18 No. 4 p.301 ~ p.305
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Pancreatin Production by Removal of Lipid from Hog Pancreas using Supercritical Carbon Dioxide with Entrainer
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Kwon Hyuk-Su
Park Sun-Young
Chun Byung-soo
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Abstract
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The study of pancreatin extraction was investigated by supercritical fluid process. Using supercritical carbon dioxide extraction with entrainer the purification of pancreatin was possible to remove lipids from Hog pancreas. To observe the optimum conditions different experimental variables were changed as pressure, temperature, flow rate of solvent and 0.25 mm of sample size were evaluated for effective removal of lipids. Ethanol and n-hexane were used as an entrainer with 5 mL/min. Increasing pressure at constant temperature the efficiency of the lipid removal in Hog pancreas was improved and the protein was concentrated without denaturalization, compared that of the control Hog pancreas. The most efficient conditions of lipid elimination were 17 MPa of pressure and 35 of temperature and 0.25 mm of sample size.
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KEYWORD
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Pancreas, pancreatin, supercritical carbon dioxide
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